Transgene detection

Model：FT-YX416

Brand：fengtu

Transgene detection utilizes rapid extraction reagents to extract genomic DNA from samples in one step for rapid amplification and real-time fluorescence detection....

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1、Transgene detection Product Overview

Transgene detection utilizes rapid extraction reagents to extract genomic DNA from samples in one step for rapid amplification and real-time fluorescence detection.Transgene detection is specifically designed for agricultural products such as soybeans, corn, and rice. Based on real-time fluorescence quantitative technology, it provides rapid and accurate detection of genetically modified components. Portable and easy to use, it delivers quick results and is suitable for field use and seed screening, providing reliable support for compliance and quality control in agriculture.

2、Transgene detection System Components

Transgene detection consists of a four-channel, 16-well fluorescence quantitative PCR instrument and a portable accessory case.

3、Transgene detection Technical Specifications

1. Features a high-grade aluminum alloy sample holder with excellent thermal conductivity, corrosion resistance, and oxidation resistance, along with Peltier heating and temperature control, and a comprehensive sealing design. Neither sample solution nor condensation will leak into the Peltier temperature control element or other electronic components below the sample holder, effectively extending the instrument's lifespan.

2. Utilizes a high-precision PT1000 temperature sensor, achieving a temperature control accuracy of 0.01℃, ensuring precise temperature control.

3. Sample throughput: 0.2ml single tube, 0.2ml 8-tube strip.

4. Open reagent and consumable system: Compatible with reagents from any brand, suitable for standard 8-tube strips or 0.2ml PCR single tubes.

5. Employs four high-intensity, broad-spectrum, long-life LED solid-state light sources (blue, green, red, and yellow), ensuring strong light intensity across the entire spectral detection range, improving detection sensitivity. These LEDs generate no heat, have a long lifespan, and are maintenance-free for life.

6. High-sensitivity detector: Uses a high-sensitivity photomultiplier tube imported from Japan for higher detection sensitivity.

7. No optical path correction required: The scanning system ensures that the signal excitation and detection optical paths are completely consistent for each well, eliminating the need for optical path correction. This frees up a channel for correction, enabling true four-channel gene detection. ★

8. Number of channels: 4 channels.

9. Channel crosstalk: Uses high-precision narrow-band filters to greatly reduce channel crosstalk, and the software can also correct crosstalk parameters. 10. Detection Sensitivity: Capable of detecting single-copy DNA templates, and clearly distinguishing between 1000 and 2000 copies of plasmid DNA.

11. Linear Detection Range: 10 orders of magnitude ★

12. Supports common fluorescent dyes and probe detection methods, such as SYBR Green I, Eva Green, Taqman, FRET, Molecular Beacons, Scorpions, Amplifluor, etc.

13. Module Temperature Range: 4-99℃

14. Control Mode: Built-in PCR operating system on the system screen or Windows computer ★

15. Interface: USB connection to computer

16. Operating Noise: Silent technology, maximum operating noise does not exceed 60dB during operation.

17. Operating and Analysis Software

17.1 Allows defining sample wells after running the program, saving experimental time.

17.2 Allows opening other experimental data results for review and analysis simultaneously while running the program.

17.3 The software automatically calculates the baseline range and sets the threshold line, providing users with reliable and accurate data results.

17.4 Experimental program settings and detection results can be directly printed as an experimental report, supporting export in multiple file formats and data styles such as XLS, CSV, and WORD ★

17.5 Results from the same batch of experiments can be grouped for independent data analysis, with a maximum of 2 groups.

17.6 Provides both Chinese and English software interfaces. ★

17.7 The software can be installed on multiple computers without limitation, no purchase of usage rights is required, and lifetime free upgrades are available.

Transgene detection Instrument Technical Specifications ★

Basic performance
Dimensions	270352195(mm)
Weight	7.1kg
Power supply	220V
Power	Maximum working power 300w
Operating system	Comes with Android operating system
External computer	External Windows computer (Win7 system and above)
System language	Real-time switch between Chinese and English
Communication interface	Square USB
Operating environment parameters
Operating ambient temperature	18～35℃
Operating environment relative humidity	0%～85%
Transport and storage temperature	-20～55℃
Transport and storage relative humidity	0%～85%
System performance
Sample size	16 holes*0.2ml
Sample volume	15～100ul
Applicable consumables	0.2ml single tube; 8×0.2ml rack tube
Temperature control range	4～99℃
Hot cover temperature	30℃-108℃(default 105℃, hot plate temperature adjustable)
Temperature control method	High-performance imported semiconductors
Gradient temperature control	3-module gradient temperature control, maximum temperature difference support 35℃
Rise rate	4.5℃/s(MAX)
Cooling rate	3.4℃/s(MAX)
Temperature control accuracy	±0.1℃
Temperature homogeneity	±0.25℃
Temperature accuracy	±0.1℃
Functional operation	Pre-set projects and analysis parameters, easy to operate
Report	Support report custom modules, 16-hole sub-project printing
Analytical function	Multiple qPCR data analysis functions, can detect 4 detection channels at the same time
Power-off protection	Can restart interrupted experiments with one key.
Instrument information	Have fault records, analysis, prompt functions, more convenient remote problem solving.
Analysis algorithm	Amplification curve, standard curve quantitative, melting curve, gene typing (SNP), relative quantification
Support for HRM detection	Support
Data Export	The experimental results can be directly exported in the form of graphics and tables; the detailed content of the data report includes experimental conditions, data coordinates and tables, and data analysis parameters.
Data format	XLS, CSV, DOCX, PDF
TouchDown function	Support time and temperature increase and decrease mode
Fluorescence detection system performance
Detection sensitivity	Detectable single copy genes
Dynamic range	1~10^10
Fluorescence repeatability	CV<1% (using a certain brand of reagent for a 16-well uniformity test, the Ct difference between 16 wells is kept within 0.5, and the CV is kept within 1%).
Correlation coefficient	0.99~1 (three replicates for each concentration, the correlation coefficient of the standard curve >0.99, the amplification efficiency was maintained between 99%~101%)
Amplification efficiency	99%~101%
Credibility	Distinguish 1000 from 2000 copy concentration differences
Laser light source	High power LED (maintenance free, long life)
Detector	High sensitivity photoelectric sensor
Excite and detect the transmission medium	High temperature resistant professional optical fiber
Excitation wavelength	460nm~480nm, 525nm~545nm, 575nm~595nm, 600nm~620nm
Detect light wavelength	505nm~525nm; 555nm~575nm; 600nm~620nm; 660nm~680nm