African swine fever laboratory diagnostics

1、African swine fever laboratory diagnostics Instrument Application

African swine fever laboratory diagnostics is widely used for the detection of animal origin ingredients in meat and African swine fever detection.African Swine Fever (ASF) virus detection is a crucial measure in ASF prevention and control, and is of great significance. To further improve the accuracy of ASF virus detection results and standardize the production, distribution, and use of ASF virus diagnostic products, from January 1, 2021, all relevant departments and units conducting ASF virus detection on pigs and their products during animal quarantine or disease monitoring and diagnosis must use ASF virus diagnostic products that have obtained product approval numbers issued by the Ministry of Agriculture and Rural Affairs to ensure accurate test results.

African swine fever laboratory diagnostics (real-time fluorescence quantitative PCR instrument supporting variable temperature detection) is used to run virus detection experiments and analyze experimental data; the instrument can be operated both in the laboratory and for field scientific experiments.  With appropriate reagents, it can perform rapid and accurate qualitative detection of target nucleic acids in analytes or other analytes from samples to be tested.

African swine fever laboratory diagnostics instrument, along with the accompanying African Swine Fever virus fluorescence PCR detection kit and African Swine Fever virus fluorescence PCR nucleic acid detection kit, have both received product approval from the Ministry of Agriculture and Rural Affairs, meeting the needs for rapid on-site detection of African Swine Fever nucleic acid. It can quantitatively and rapidly diagnose livestock diseases such as African Swine Fever, avian influenza, classical swine fever, porcine reproductive and respiratory syndrome (PRRS), and pseudorabies, and is widely used in farms, slaughterhouses, food processing plants, meat processing enterprises, the Ministry of Agriculture and Rural Affairs, animal husbandry bureaus, and inspection and quarantine units.

Experimenters need to undergo specialized training in laboratory techniques and instrument and software operation, and possess proficient relevant operating skills.

System composition: Dual-channel 16-well fluorescence quantitative PCR instrument, portable accessory box.

2、African swine fever laboratory diagnostics Product Advantages

1. Intelligent Operating System, Complete Control Over Experiments

A. Program Updates and Presets: Supports online updates of experimental programs and includes a wealth of built-in preset experimental program templates. Users can also customize and save frequently used templates for quick one-click startup, significantly improving operational efficiency.

B. Simplified Startup Experience (Optional): A customizable integrated camera scanning system allows users to simply scan the QR code on the sample plate or program card to automatically identify and load the corresponding experimental program, simplifying the process and avoiding human error.

C. Easy Operation: The touchscreen interface is intuitive and easy to understand, allowing new users to quickly get started. Pre-installed common program templates further simplify the operation process.

2. Precise and Stable Temperature Control and Optical Core

A. Long-lasting and Precise Temperature Control System: Uses imported high-quality TEC semiconductor cooling modules for precise temperature control (±0.1°C) and excellent temperature uniformity between wells (≤±0.3°C). The system boasts exceptional stability, minimal performance degradation over long-term use, and a calibration cycle of several years, significantly reducing maintenance costs and operational downtime.

B. Maintenance-Free and Sensitive Optical System: Equipped with two independent LED light sources and two independent photodiode detectors, enabling simultaneous and precise dual-channel fluorescence detection. Uses ultra-long-life imported light sources, requiring no maintenance, calibration, or replacement during its design lifespan, ensuring long-term consistency and high sensitivity of detection results.

C. Excellent Data Quality: High experimental linearity (R²≥0.999) and excellent repeatability (low CV value between wells) ensure accurate and reliable quantitative results and high data confidence.

3. Efficient and Convenient Data and Output Management

A. Multiple Data Export Methods: Supports direct copying via USB storage devices or data transfer to local servers or the cloud via Wi-Fi wireless network, offering convenience and flexibility to meet various laboratory data management needs. (Optional)

B. Rich Report Export and Printing Options: Supports generating and exporting experimental reports in various formats (such as PDF and Excel). The instrument is compatible with USB thermal printers, Bluetooth wireless printers, and standard A4 printers, allowing for one-click export and printing of professional reports directly from the instrument or via a computer, facilitating result archiving and instant sharing. 4. Fast, Efficient, and Durable

A. Rapid Heating and Cooling: The optimized temperature control system features high-speed heating and cooling capabilities, significantly shortening single-cycle and overall testing times, and increasing experimental throughput.

B. Extended Service Life: All core components are made of industrial-grade durable materials and designed for long-term reliability, ensuring a lasting return on investment for users.

3、African swine fever laboratory diagnostics Technical Specifications

1. Features a high-grade aluminum alloy sample holder with excellent thermal conductivity, corrosion resistance, and oxidation resistance, along with Peltier heating and temperature control, and a comprehensive sealing design. Neither sample solution nor condensed water will leak into the Peltier temperature control element or other electronic components below the sample holder, effectively extending the instrument's lifespan.

2. Utilizes a high-precision PT1000 temperature sensor, achieving a temperature control accuracy of 0.01℃, ensuring accurate temperature control.

3. Sample throughput: 0.2ml single tube, 0.2ml 8-tube strip.

4. Open reagent and consumable system: Compatible with reagents from any brand, suitable for standard 8-tube strips or 0.2ml PCR single tubes.

5. Employs blue and green high-intensity, broad-spectrum, long-life LED solid-state light sources, ensuring strong light intensity across the entire spectral detection range, improving detection sensitivity.  These LEDs also generate no heat, have a long lifespan, and are maintenance-free for life.

6. High-sensitivity detector: Uses a high-sensitivity photomultiplier tube imported from Japan for higher detection sensitivity.

7. No optical path correction required: The scanning system ensures that the signal excitation and detection optical paths are completely consistent for each well, eliminating the need for optical path correction. ★

8. Number of channels: Configured with dual channels.

9. Channel crosstalk: Uses high-precision narrow-band filters to greatly reduce channel crosstalk, and the software can also correct crosstalk parameters. 10. Detection Sensitivity: Capable of detecting single-copy DNA templates and clearly distinguishing between 1000 and 2000 copies of plasmid DNA.

11. Linear Detection Range: 10 orders of magnitude ★

12. Supports common fluorescent dyes and probe detection methods, such as FAM, VIC, etc.

13. Module Temperature Range: 4-99℃

14. Control Mode: Built-in PCR operating system on the system screen; 1.14 Interface: Computer USB connection ★

15. Operating Noise: Silent technology, maximum operating noise does not exceed 60dB during operation.

16. Operating and Analysis Software

16.1 Allows defining sample wells after running the program, saving experimental time.

16.2 The software automatically calculates the baseline range and sets the threshold line, providing users with reliable and accurate data results.

16.3 Experimental program settings and detection results can be directly printed as an experimental report, supporting various file formats and data styles such as XLS, CSV, and WORD for export. ★

16.4 Provides both Chinese and English software interfaces. ★

African swine fever laboratory diagnostics Instrument Technical Specifications