African Swine Fever Virus

1、African Swine Fever Virus Instrument Application

African Swine Fever Virus uses PCR technology to determine whether the virus is infected by detecting the viral nucleic acid in the blood, saliva and other samples of pets.African Swine Fever (ASF) virus detection is a crucial measure in ASF prevention and control efforts, and is of great significance. To further improve the accuracy of ASF virus detection results and standardize the production, distribution, and use of ASF virus diagnostic products, from January 1, 2021, all relevant departments and units conducting ASF virus detection on pigs and their products during animal quarantine or disease monitoring and diagnosis should use ASF virus diagnostic products that have obtained product approval numbers issued by the Ministry of Agriculture and Rural Affairs to ensure accurate test results.

African Swine Fever Virus (real-time fluorescence quantitative PCR instrument supporting variable temperature detection) is used to run virus detection experiments and analyze experimental data; the instrument can be operated both in the laboratory and for field scientific experiments.  With appropriate reagents, it can perform rapid and accurate qualitative detection of target nucleic acids in analytes or other analytes from samples to be tested.

African Swine Fever Virus is compatible with African Swine Fever virus fluorescence PCR detection kits and African Swine Fever virus fluorescence PCR nucleic acid detection kits, both of which have obtained product approval from the Ministry of Agriculture and Rural Affairs, and can meet the needs of rapid on-site detection of African Swine Fever nucleic acid. It can quantitatively and rapidly diagnose livestock diseases such as African Swine Fever, avian influenza, classical swine fever, porcine reproductive and respiratory syndrome, and pseudorabies. It is widely used in farms, slaughterhouses, food processing plants, meat processing enterprises, the Ministry of Agriculture and Rural Affairs, animal husbandry bureaus, and inspection and quarantine units.

Experimenters need to undergo specialized training in laboratory technology and instrument and software operation, and possess proficient relevant operating skills.

System composition: Four-channel 32-well fluorescence quantitative PCR instrument, portable accessory box

2、African Swine Fever Virus Product Advantages

1. Intelligent Operating System, Complete Control Over Experiments

A. Flexible Dual-Mode Control: Supports independent operation via the instrument's touchscreen and remote control and data management via computer software, adapting to different experimental scenarios.

B. Program Updates and Presets: Supports online updates of experimental programs and includes a wealth of built-in preset experimental program templates. Users can also customize and save frequently used templates for quick one-click startup, significantly improving operational efficiency.

C. Simplified Startup Experience (Optional): A customizable integrated camera scanning system allows users to automatically identify and load the corresponding experimental program by simply scanning the QR code on the sample plate or program card, simplifying the process and avoiding human error.

D. Easy Operation: Both the touchscreen interface and computer software are designed to be intuitive and easy to understand, allowing new users to quickly get started. Pre-installed common program templates further simplify the operation process.

2. Precise and Stable Temperature Control and Optical Core

A. Long-Term Precise Temperature Control System: Uses imported high-quality TEC semiconductor cooling modules for precise temperature control (±0.1°C) and excellent temperature uniformity between wells (≤±0.3°C). The system boasts excellent stability, minimal performance degradation over long-term use, and a calibration cycle of several years, significantly reducing maintenance costs and operational downtime.

B. Maintenance-Free and Sensitive Optical System: Equipped with 4 independent LED light sources and 4 independent photodiode detectors, enabling simultaneous and precise detection of four fluorescence channels. Uses ultra-long-life imported light sources, requiring no maintenance, calibration, or replacement during its design lifespan, ensuring long-term consistency and high sensitivity of detection results.

C. Excellent Data Quality: High experimental linearity (R²≥0.999) and excellent repeatability (low CV value between wells) ensure accurate and reliable quantitative results and high data confidence.

3. Efficient and Convenient Data and Output Management

A. Multiple Data Export Methods: Supports direct copying via USB storage devices or data transfer to local servers or the cloud via Wi-Fi wireless network, providing convenience and flexibility to meet various laboratory data management habits.

B. Rich Report Export and Printing: Supports generating and exporting experimental reports in various formats (such as PDF and Excel). The instrument is compatible with USB thermal printers, Bluetooth wireless printers, and standard A4 printers, allowing for one-click export and printing of professional reports directly from the instrument or via a computer, facilitating result archiving and instant sharing. 4. Fast, Efficient, and Durable

A. Rapid Heating and Cooling: The optimized temperature control system features high-speed heating and cooling capabilities, significantly shortening single-cycle and overall testing times, and increasing experimental throughput.

B. Extended Service Life: All core components are made of industrial-grade durable materials and designed for reliability, ensuring stable and dependable operation and providing users with a long-term return on investment.

3、African Swine Fever Virus Technical Specifications

1. Features a high-grade aluminum alloy sample holder with excellent thermal conductivity, corrosion resistance, and oxidation resistance, along with Peltier heating and temperature control, and a comprehensive sealing design. Neither sample solution nor condensation will leak into the Peltier temperature control element or other electronic components below the sample holder, effectively extending the instrument's lifespan.

2. Utilizes a high-precision PT1000 temperature sensor, achieving a temperature control accuracy of 0.01℃, ensuring precise temperature control.

3. Sample throughput: 0.2ml single tube, 0.2ml 8-tube strip.

4. Open reagent and consumable system: Compatible with reagents from any brand, suitable for standard 8-tube strips or 0.2ml PCR single tubes.

5. Employs four high-intensity, broad-spectrum, long-life LED solid-state light sources (blue, green, red, and yellow), ensuring strong light intensity across the entire spectral detection range, improving detection sensitivity.  These LEDs generate no heat, have a long lifespan, and are maintenance-free for life.

6. High-sensitivity detector: Uses a high-sensitivity photomultiplier tube imported from Japan for higher detection sensitivity.

7. No optical path correction required: The scanning system ensures that the signal excitation and detection optical paths are completely consistent for each well, eliminating the need for optical path correction. This frees up a channel for correction, enabling true four-channel gene detection. ★

8. Number of channels: 4 channels.

9. Channel crosstalk: Uses high-precision narrow-band filters to greatly reduce channel crosstalk, and the software can also correct crosstalk parameters. 10. Detection Sensitivity: Capable of detecting single-copy DNA templates and clearly distinguishing between 1000 and 2000 copies of plasmid DNA.

11. Linear Detection Range: 10 orders of magnitude ★

12. Supports common fluorescent dyes and probe detection methods, such as SYBR Green I, Eva Green, Taqman, FRET, Molecular Beacons, Scorpions, Amplifluor, etc.

13. Module Temperature Range: 4-99℃

14. Control Mode: Built-in PCR operating system on the system screen or Windows computer ★

15. Interface: USB connection to computer

16. Operating Noise: Silent technology, maximum operating noise does not exceed 60dB during operation.

17. Operating and Analysis Software

17.1 Allows defining sample wells after running the program, saving experimental time.

17.2 Allows opening other experimental data results for viewing and analysis simultaneously while running the program.

17.3 The software automatically calculates the baseline range and sets the threshold line, providing users with reliable and accurate data results.

17.4 Experimental program settings and detection results can be directly printed as an experimental report, supporting export in multiple file formats and data styles such as XLS, CSV, and WORD ★

17.5 Results from the same batch of experiments can be grouped for independent data analysis, with up to 4 groups possible.

17.6 Provides both Chinese and English software interfaces. ★

17.7 The software can be installed on multiple computers without limitation, requiring no payment for usage rights, and offers free lifetime upgrades. ★

African Swine Fever Virus Instrument Technical Specifications