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Fluorescence Quantitative PCR Detection System application scenarios:
Fluorescence Quantitative PCR Detection System has four fluorescence detection channels and can detect 96 samples at a time, which can meet most experimental needs.
basic scientific research
Pathogen detection
Adulteration of meat products
GMO testing
Food safety testing
Drug development and rational drug use
gene expression
water body monitoring
Fluorescence Quantitative PCR Detection System Instrument Technical Specifications
Basic performance | |
Overall dimensions | 410*320*196 mm |
weight | 11kg |
power supply | 220V |
Communication interface | USB |
Operating environment parameters | |
Operating ambient temperature | 18~35℃ |
Operating environment relative humidity | 0%~85% |
Transport and storage temperature | -20~55℃ |
Transport and storage relative humidity | 0%~85% |
System performance | |
Sample capacity | 96 holes*0.2ml |
Sample volume | 20~120ul |
Applicable consumables | 0.2ml single tube, 8×0.2ml row tubes |
Temperature control range | 4~99℃ |
Temperature control accuracy | ±0.1℃ |
average heating rate | 3.5℃/s |
average cooling rate | 3°C/s |
Fluorescence detection system performance | |
light source | High brightness LED |
detector | photomultiplier tube |
Excitation light wavelength | 460nm~480nm, 525nm~545nm, 575nm~595nm, 600nm~620nm |
Detection light wavelength | 505nm~525nm, 555nm~575nm, 600nm~620nm, 660nm~680nm |
Main technical parameters of Fluorescence Quantitative PCR Detection System (★ represents parameters that must be met)
★1.1 Sample tank: Using high-standard aluminum alloy sample tank, it has good thermal conductivity, corrosion resistance and oxidation resistance, Peltier heating and temperature control, and comprehensive sealing design. Neither sample solution nor condensed water will leak into the Peltier temperature control element and other electronic components under the sample tank, which can effectively extend the service life of the instrument.
★1.2 Precise temperature control system: Using high-precision PT1000 temperature sensor, the temperature control accuracy reaches 0.01℃, fully ensuring the accuracy of temperature control.
1.3 Sample throughput: 96×0.2ml PCR tube, 96-well plate, 12×8 tube strip.
1.4 Open reagent and consumable system: You can choose any brand of reagents, suitable for standard 96-well plates, 8-tube strips or 0.2ml PCR single tubes
★1.5 High-intensity and wide-spectrum excitation light source: four high-intensity and long-life LED solid-state light sources of blue, green, red and yellow are used to ensure strong light intensity in the entire spectrum detection range, improve detection sensitivity, and do not Heat, long service life, maintenance-free for life
1.6 High-sensitivity detector: Using high-sensitivity photomultiplier tubes imported from Japan, the detection sensitivity is higher
1.7 No need for optical path correction: The scanning system can ensure that the signal excitation and detection light path of each well are completely consistent, without the need for optical path correction, so there is no need to occupy an additional correction channel, and true four-channel genetic detection can be achieved
★1.8 Number of channels: Configure 4 channels.
1.8.1 Channel crosstalk: Using high-precision narrow-band filters to greatly reduce channel crosstalk, the software can also correct crosstalk parameters
★1.9 Detection sensitivity: Able to detect single copy of DNA template and clearly distinguish between 1000 copies and 2000 copies of plasmid DNA
★1.10 Linear detection range: 10 orders of magnitude
1.11 Available technical methods: Supports commonly used fluorescent dye and probe detection methods, such as SYBR Green I, Eva Green, Taqman, FRET, Molecular Beacons, Scorpions, Amplifluor, etc.
1.12 Module temperature range: 4 - 99 ℃
1.13 Control mode: Windows computer
1.14 interface: computer USB connection
1.15 Working noise: Silent technology, the maximum working noise during operation does not exceed 45 dB
1.16 Operation analysis software
1.16.1 You can define sample holes after running the program to save experimental time
1.16.2 When running the program, you can open other experimental data results for review and analysis.
1.16.3 The software automatically calculates the baseline range and automatically sets the threshold line, providing users with reliable and accurate data results.
★1.16.4 The experimental program setting information and test results can be directly printed into experimental reports, and support the export of multiple file formats and data styles such as XLS, CSV, WORD, etc.
1.16.5 The results of the same batch of experiments can be grouped for independent data analysis, up to 12 groups
★1.16.6 provides Chinese and English software interfaces.
★The 1.16.7 software can be installed on multiple computers without any restrictions. There is no need to pay for the right to use it, and it can be upgraded for free for life.
2Basic configuration:
2.1 Quantitative PCR instrument host
2.3 Operating Instructions
2.4 Power cable and data cable
2.5 A set of operation analysis software